Browse by Year:
2025
Jangra, M., Travin, D.Y., Aleksandrova, E.V., Kaur, M., Darwish, L., Koteva, K., Klepacki, D., Wang, W., Tiffany, M., Sokaribo, A., Coombes, B. K., Vázquez-Laslop, N., Polikanov, Y. S., Mankin, A. S., & Wright. G. D. (2025) A broad-spectrum lasso peptide antibiotic targeting the bacterial ribosome. (2025) Nature 640:1022–1030
Syroegin, E.A., Aleksandrova, E.V., Kruglov, A.A., Paranjpe, M.N., Svetlov, M.S., & Polikanov, Y.S. Structural insights into context-specific inhibition of bacterial translation by macrolides. Nat Commun 16, 9685 (2025).
2024
Koller, T.O., Berger, M. J., Morici, M., Paternoga, H., Bulatov, T., Di Stasi, A., Dang, T., Mainz, A., Raulf, K., Crowe-McAuliffe, C., Scocchi, M., Mardirossian, M., Beckert, B., Vázquez-Laslop, N., Mankin, A. S., Süssmuth, R.D., & Wilson D. N. Paenilamicins are context-specific translocation inhibitors of protein synthesis. (2024) Nat. Chem. Biol. 20(12):1691-1700
Aleksandrova, E. V., Ma, C. X., Klepacki, D., Alizadeh, F., Vázquez-Laslop, N., Liang, J. H., Polikanov, Y. S., Mankin, A. S. (2024). Macrolones target bacterial ribosomes and DNA gyrase and can evade resistance mechanisms. Nat. Chem. Biol. 20(12):1680-1690
Huang, W., Baliga, C., Aleksandrova, E. V., Atkinson G.C., Polikanov, Y. S. , Vázquez-Laslop, N., Mankin A. S. (2024). Activity, structure, and diversity of Type II proline-rich antimicrobial peptides from insects. EMBO Rep, 25(11):5194-5211
Huang, W., Baliga, C., Vázquez-Laslop, N., Mankin, A.S. (2024) Sequence diversity of apidaecin-like peptides arresting the terminating ribosome. (2024) Nucleic Acids Res. 52 (15) 8967–8978
Svetlov, M.S., Dunand, C.F., Nakamoto, J.A., Atkinson, G.C., Safdari, H.A., Wilson, D.N., Vázquez-Laslop, N., Mankin, A.S., (2024) Peptidyl-tRNA hydrolase is the nascent chain release factor in bacterial ribosome-associated quality control. Mol Cell. 84, 715-726.
Judd, H.N.G., Martínez, A.K., Klepacki, D., Vázquez-Laslop, N., Sachs, M.S., Cruz-Vera, L.R. (2024) Functional domains of a ribosome arresting peptide are affected by surrounding nonconserved residues. J. Biol. Chem. 300, 105780
2023
Skowron, K. J., Baliga, C., Johnson, T., Kremiller, K. M., Castroverde, A., Dean, T. T., Allen, A. C., Lopez-Hernandez, A. M., Aleksandrova, E. V., Klepacki, D., Mankin, A. S., Polikanov, Y. S., Moore, T. W. (2023). Structure-activity relationships of the antimicrobial peptide natural product apidaecin. J. Med, Chem., 66, 11831–11842.
Vàzquez-Laslop, N. & Polikanov, Y.S. (2023) Motif-ation matters. Nat. Chem. Biol. 19, 1044-1045
Wu, K. J. Y., Klepacki, D., Mankin, A. S., Myers, A. G. (2023). A method for tritiation of iboxamycin permits measurement of its ribosomal binding. Bioorg. & Med. Chem. Lett., 91, 129364.
Mangano, K., Klepacki, D., Ohanmu, I., Baliga, C., Huang, W., Brakel, A., Krizsan, A., Polikanov, Y. S., Hoffmann, R., Vázquez-Laslop, N., & Mankin, A. S. (2023). Inhibition of translation termination by the antimicrobial peptide Drosocin. Nat. Chem. Biol., 19, 1082–1090.
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- Zhang, Y., Aleksashin, N. A., Klepacki, D. Anderson, C., Vazquez-Laslop, N., Gross, C. A., Mankin, A.S. (2022) The context of the ribosome binding site in mRNAs defines specificity of action of kasugamycin, an inhibitor of translation initiation Proc. Natl. Acad. Sci. USA 119, e2118553119.
- Tsai, K., Stojkovic, V., Lee, D.J., Young, I.D., Szal, T., Klepacki, D., Vázquez-Laslop, N., Mankin, A.S., Fraser, J.S., Fujimori, D.G. (2022) Structural basis for context-specific inhibition of translation by oxazolidinone antibiotics Nat. Struct. Molec. Biol. 29, 162-171.
- Syroegin, E.A., Flemmich, L., Klepacki, D., Vázquez-Laslop, N., Micura, R., Polikanov, Y.S. (2022) Structural basis for the context-specific action of the classic peptidyl transferase inhibitor chloramphenicol Nat. Struct. Molec. Biol. 29, 152-161.
- Vázquez-Laslop, N., Shrama, C.M., Mankin, A.S., Buskirk, A.R. (2022) Identifying Small Open Reading Frames in Prokaryotes with Ribosome Profiling J. Bacteriol. 204, e00294-21.
- Mangano, K., Marks, J., Klepacki, D., Saha, C.K., Atkinson, G.C., Vázquez-Laslop, N., Mankin, A.S. (2022) Context-based sensing of orthosomycin antibiotics by the translating ribosome Nature Chem Biol 18, 1277-1286.
- Laczkovich, I., Mangano, K., Shao, X., Hockenberry, A.J., Gao, Y., Mankin, A., Vazquez-Laslop, N., and Federle, M.J. (2022) Discovery of unannotated small open reading rrames in Streptococcus pneumoniae D39 Involved in quorum sensing and virulence using ribosome profiling mBio 13, e0124722.
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- Svetlov, M.S, Syroegin, E.A., Aleksandrova, E.V., Atkinson, G.C., Gregory, S.T., Mankin, A.S. Polikanov, Y.S. (2021) Structure of Erm-modified 70S ribosome reveals the mechanism of macrolide resistance Nat.Chem.Biol. 17, 412-420.
- Svetlov, M.S., Koller, T.O., Meydan, S., Shankar, V., Klepacki, D., Polacek, N., Guydosh, N.R., Vázquez-Laslop, N., Wilson, D.N., Mankin, A.S. (2021) Context-specific action of macrolide antibiotics on the eukaryotic ribosome Nat Commun 12, 2803.
- Meydan, S., Klepacki, D., Mankin, A.S., Vázquez-Laslop, N. (2021) Identification of translation start sites in bacterial genomes. Methods in Molecular Biology 2252, 27-55.
- Baliga, C., Brown, T.J., Florin, T., COlon, S., Shah, V., Skowron, K.J., Kefi, A., Szal, T., Klepacki, D., Moore, T.W., Vázquez-Laslop, N., Mankin, A.S. (2021) Charting the sequence-activity landscape of peptide inhibitors of translation termination Proc Natl Acad Sci USA 118, e2026465118.
- Beckert, B., Leroy, E.C., Sothiselvam, S., Bock, L.V., Svetlov. M.S., Graf, M., Arenz, S., Abdelshahid, M., Seip, B., Grubmüller, H., Mankin, A.S., Innis, C.A., Vázquez-Laslop, N., Wilson, D.N. (2021) Structural and mechanistic basis for translation inhibition by macrolide and ketolide antibiotics Nat Commun 12, 4466.
- van der Stel, A.-X., Gordon, E.R., Sengupta, A., Martinez, A.K., Klepacki, D., Perry, T.N., del Valle, A.H., Vázquez-Laslop, N., Sachs, M.S., Cruz-Vera, L.R., Innis, C.A. (2021) Structural basis for the tryptophan sensitivity of TnaC-mediated ribosome stalling Nat Commun 12, 5340.
- Mitcheltree, M.J., Pisipati, A., Syroegin, E.A., Silvestre, K.J., Klepacki, D., Mason, J.D., Terwilliger, D.W., Testolin, G., Pote, A.R., Wu, K.J.Y., Ladley, R.P., Chatman, K., Mankin, A.S., Polikanov, Y.S., Myers, A.G. (2021) A synthetic antibiotic class overcoming bacterial multidrug resistance Nature 599, 507-512.
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- Mangano, K., Florin, T., Shao, X., Klepacki, D., Chelysheva, I., Ignatova, Z., Gao, Y., Mankin, A.S., Vázquez-Laslop, N. (2020) Genome-wide effects of the antimicrobial peptide apidaecin on translation termination in bacteria eLife 0, .
- Svetlov, M.S., Cohen, S., Alsuhebany, N., Vázquez-Laslop, N., Mankin, A.S. (2020) A long-distance rRNA base pair impacts the ability of macrolide antibiotics to kill bacteria Proc Natl Acad Sci USA 117, 1971-1975.
- Huang, S., Aleksashin N.A., Loveland, A.B., Klepacki, D., Reier, K., Kefi, A., Szal, T., Remme, J., Jaeger, L., Vázquez-Laslop, N., Korostelev, A.A., Mankin, A.S. (2020) Ribosome engineering reveals the importance of 5S rRNA autonomy for ribosome assembly Nat Commun 10, 1038.
- Aleksashin, N.A., Szal, T., d’Aquino, A.E., Jewett, M.C., Vázquez-Laslop, N., Mankin, A.S (2020) A fully orthogonal system for protein synthesis in bacterial cells Nat Commun 11, 1858 .
- Choi, J., Marks, J., Zhang, J., Chen, D.-H., Wng, J., Vázquez-Laslop, N., Mankin, A.S., Puglisi, J.D. (2020) Dynamics of the context-specific translation arrest by chloramphenicol and linezolid Nature Chem Biol 16, 310-317.
- Ovchinnikov, S.V., Bikmetov, D., Livenskyi, A., Serebryakova, M., Wilcox, B., Mangano, K., Shiriaev, D.I., Osterman, I.A., Sergiev, P.V., Borukhov, S., Vazquez-Laslop, N., Mankin, A.S., Severinov, K., Dubiley, S. (2020) Mechanism of translation inhibition by type II GNAT toxin AtaT2 Nucleic Acids Res 48, 8617-8625.
- Mankin, A.S. (2020) Frame-shifted proteins of a given gene are unlikely to retain the same function RNA 26, 1301-1302.
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- Meydan, S., Marks, J., Klepacki, D., Sharma, V., Baranov, P.V., Firth A.E., Margus, T., Kefi, A.m, Vázquez-Laslop, N., Mankin, A.S. (2019) Retapamulin-assisted ribosome profiling reveals the alternative bacterial proteome Mol Cell 74, 1-13.
- Aleksashin, N.A., Leppik, M., Hockenberry, A.J., Klepacki, D., Vázquez-Laslop, N., Jewett, M.C., Remme, J., Mankin, A.S. (2019) Assembly and functionality of the ribosome with tethered subunits Nat Commun 10, 930.
- Carlson, E.D., d’Aquino, A.E., Kim, D.S., Fulk, E.M., Hoang, K., Szal, T., Mankin, A.S., Jewett, M.C. (2019) Engineered ribosomes with tethered subunits for expanding biological function. Nat Commun 10, 3920.
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- Meydan, S., Vázquez-Laslop, N., Mankin, A.S. (2018) Genes within genes in bacterial genomes Microbiol Spectrum 0, 0020-2018.
- Tereshchenkov, A.G., Dobosz-Bartoszek, M., Osterman, I.A., Marks, J., Sergeeva, V.A., Kasatsky, P., Komarova, E.S., Stavrianidi, A.N., Rodin, I.A., Konevega, A.L., Sergiev, P.V., Sumbatyan, N.V., Mankin, A.S., Bogdanov, A.A., Polikanov, Y.S. (2018) Binding and Action of Amino Acid Analogs of Chloramphenicol upon the Bacterial Ribosome J Mol Biol 430, 842-852.
- Pantel, L., Florin, T., Dobosz-Bartoszek, M., Racine E., Sarciaux, M., Serri, M., Houard, J., Campagne, J.M., de Figueiredo, R.M., Midrier, C., Gaudriault, S., Givaudan, A., Lanois, A., Forst, S., Aumelas, A., Cotteaux-Lautard, C., Bolla, J.M., Vingsbo Lundberg, C., Huseby, D.L., Hughes, D., Villain-Guillot, P., Mankin, A.S., Polikanov, Y.S., Gualtieri, M. (2018) Odilorhabdins, antibacterial agents that cause miscoding by binding at a new ribosomal site Mol Cell 70, 83-94.
- Vázquez-Laslop, N., Mankin, A.S. (2018) Context-specific action of ribosomal antibiotics Ann Rev Microbiol 72, 185-207.
- Vázquez-Laslop, N., Mankin, A.S. (2018) How macrolide antibiotics work Trends Biochem Sci 43, 668-684.
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- Meydan, S., Klepacki, D., Karthikeyan, S., Thomas, P., Jones, J.E., Khan, Y., Briggs, J., Dinman, J.D., Vázquez-Laslop, N., Mankin, A.S. (2017) Programmed ribosomal frameshifting generates a copper transporter and a copper chaperone from the same gene Mol Cell 65, 207-219.
- Svetlov, M.S., Vázquez-Laslop, N., Mankin, A.S. (2017) Kinetics of drug–ribosome interactions defines the cidality of macrolide antibiotics Proc Natl Acad Sci USA 114, 13673-13678.
- Almutairi, M., Svetlov, M.S., Hansen, D.A., Khabibullina, N.F., Klepacki, D., Kang, H-Y., Sherman, D.H., Vázquez-Laslop, N., Polikanov, Y.S., Mankin, A.S. (2017) Co-produced natural ketolides methymycin and pikromycin inhibit bacterial growth by preventing synthesis of a limited number of proteins Nucl Acids Res 45, 9573-9582.
- Florin, T., Maracci, C., Graf, M., Karki, P., Klepacki, D., Berninghausen, O., Beckmann, R., Vázquez-Laslop, N., Wilson, D.N., Rodnina, M.V., Mankin, A.S. (2017) An antimicrobial peptide that inhibits translation by trapping release factors on the ribosome Nat Struct Molec Biol 24, 752-757.
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- Gupta, P., Liu, B., Klepacki, D., Gupta, V., Schulten, K., Mankin, A.S. , Vázquez-Laslop, N. (2016) Nascent peptide assists the ribosome in recognizing chemically distinct small molecules Nature Chem Biol 12, 153-158.
- Sothiselvam, S., Neuner, S., Rigger, L., Klepacki, D., Micura, R., Vazquez-Laslop, N., Mankin, A.S. (2016) Binding of macrolide antibiotics compels the ribosome to discriminate against specific substrates based on their charge and size. Cell Reports 16, 1789-1799.
- Marks, J., Kannan, K., Roncase, E. J., Klepacki, D., Kefi, A., Orelle, C., Vázquez-Laslop, N., Mankin, A.S. (2016) Context-specific inhibition of translation by ribosomal antibiotics targeting the peptidyl transferase center Proc Natl Acad Sci USA 113, 12150-12155.
- Gagnon, M.G., Roy, R.N., Lomakin, I.B., Florin, T., Mankin, A.S., Steitz, T.A. (2016) Structures of proline-rich peptides bound to the ribosome reveal a common mechanism of protein synthesis inhibition Nucl Acids Res 44, 2439-2450.
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- Orelle, C., Carlson, E.D., Szal, T., Florin, T., Jewett, M.C., Mankin, A.S. (2015) Protein synthesis by ribosomes with tethered subunits Nature 524, 119-124.
- Almutairi, M.M., Park, S.R., Rose, S., Hansen, D.A., Vázquez-Laslop, N., Douthwaite, S., Sherman, D.H. and Mankin, A.S. (2015) Resistance to ketolide antibiotics by coordinated expression of rRNA methyltransferases in a bacterial producer of natural ketolides Proc Natl Acad Sci USA 112, 12956-12961.
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- Arenz, S., Ramu, H., Gupta, P., Berninghausen, O., Beckmann, R., Vázquez-Laslop, N., Mankin, A.S., Wilson, D.N. (2014) Molecular basis for erythromycin-dependent ribosome stalling during translation of the ErmBL leader peptide Nat. Commun. 5, 3501 DOI 10.138/ncomms4501.
- Vazquez-Laslop, N., Mankin, A.S. (2014) Protein accounting in the cellular economy Cell 157, 529-531.
- Sothiselvam, S., Liu, B., Han, W., Ramu, H., Klepacki, D., Atkinson, G.C., Brauer, A., Remm, M., Tenson, T., Schulten, K., Vázquez-Laslop, N., Mankin, A.S. (2014) Macrolide antibiotics allosterically predispose the ribosome for translation arrest. Proc Natl Acad Sci USA 111, 9804-9809.
- Martínez, A.K., Gordon, E., Sengupta, A., Shirole, N., Klepacki, D., Martinez-Garriga, B., Brown, L.M., Benedik, M.J., Yanofsky, C., Mankin, A.S., Vazquez-Laslop, N., Sachs, M.S., Cruz-Vera, L.R. (2014) Interactions of the TnaC nascent peptide with rRNA in the exit tunnel enable the ribosome to respond to free tryptophan Nucl Acids Res 42, 1245–1256.
- Polikanov, Y.S., Szal, T., Jiang, F., Matsuda, R., Shiozuka, M., Steitz, T.A., Vazquez-Laslop, N., Mankin, A.S. (2014) Negamycin interferes with decoding and translocation by simultaneous interaction with rRNA and tRNA Mol Cell 56, 541-550.
- Arenz, S., Meydan, S., Starosta, A., Berninghausen, O., Beckmann, R., Vázquez-Laslop, N., Wilson, D.N. (2014) Drug sensing by the ribosome induces translational arrest via active site perturbation Mol Cell 56, 446-452.
- Kannan, K., Kanabar, P., Schryer, D., Florin, T., Oh, E., Bahroos, N., Tenson, T., Weissman, J.S., Mankin, A.S. (2014) The general mode of translation inhibition by macrolide antibiotics Proc Natl Acad Sci USA 111, 15958–15963.
- Polikanov, Y.S., Osterman, I.A., Szal, T., Tashlitsky, V.N., Serebryakova, M.V., Kusochek, P., Bulkley, D., Malanicheva, I.A., Efimenko, T.A., Efremenkova, O.V., Konevega, A.L., Shaw, K.J., Bogdanov, A.A., Rodnina, M.V., Dontsova, O.A., Mankin, A.S., Steitz, T.A., Sergiev, P.V. (2014) Amicoumacin A inhibits translation by stabilizing mRNA interaction with the ribosome Mol Cell 56, 531-540.
- Vázquez-Laslop, N., Mankin, A.S. (2014) Triggering peptide-dependent translation arrest by small molecules: ribosome stalling modulated by antibiotics. In: Regulatory Nascent Polypeptides (Ito, K., ed). Springer (Tokyo) 0, 165-186.
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- LaMarre, J., Mendes, R.E., Szal, T., Schwarz, S., Jones, R.N., Mankin, A.S. (2013) The genetic environment of the cfr gene and the presence of other mechanisms account for the very high linezolid resistance of Staphylococcus epidermidis isolate 426-3147L Antimicrob Agents Chemother 57, 1173-1179.
- Orelle, C., Szal, T., Klepacki, D., Shaw, K.J., Vázquez-Laslop, N., Mankin, A.S. (2013) Identifying the targets of aminoacyl-tRNA synthetase inhibitors by primer extension inhibition Nucl Acids Res 10, 526.
- Gupta, P., Sothiselvam, S., Vázquez-Laslop, N., Mankin, A.S. (2013) Deregulation of translation due to post-transcriptional modification of rRNA explains why erm genes are inducible Nat Commun 4, 1984 DOI: 10.1038/ncomms2984.
- Orelle, C., Carlson, S., Kaushal, B., Almutairi, M.M., Liu, H., Ochabowicz, A., Quan, S., Pham, V.C., Squires, C.L., Murphy, B.T., Mankin, A.S. (2013) Tools for characterizing bacterial protein synthesis inhibitors. Antimicrob Agents Chemother 57, 5994-6004.
- Gupta, P., Kannan, K., Mankin, A.S., Vazquez-Laslop, N. (2013) Regulation of gene expression by macrolide-induced ribosomal frameshifting Mol Cell 52, 629-642.
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- Locke JB, Rahawi S, Lamarre J, Mankin AS, Shaw KJ. (2012) Genetic environment and stability of cfr in methicillin-resistant Staphylococcus aureus CM05. Antimicrob Agents Chemother 56, 332-340.
- LeTourneau, N., Vimal, P., Klepacki, D., Mankin, A., Melman, A, (2012) Synthesis and antibacterial activity of desosamine-modified macrolide derivatives Bioorg Med Chem Lett 22, 4575-4578.
- Kannan, K., Vazquez-Laslop, N., Mankin, A.S. (2012) Selective protein synthesis by ribosomes with a drug-obstructed exit tunnel Cell 151, 508-520.
- Subramanian, S.L., Ramu, H., Mankin, A. S. (2012) Inducible Resistance to Macrolide Antibiotics Antibiotic Discovery and Development 1, 455-484.
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- LaMarre, J. M, Howden, B. P., Mankin, A. S. (2011) Inactivation of the indigenous methyltransferase RlmN in Staphylococcus aureus increases linezolid resistance Antimicrob Agents Chemother 55, 2989-2991.
- LaMarre, J. M., Locke, J.B., Shaw, K.J., Mankin, A. S. (2011) Low Fitness Cost of the Multidrug Resistance Gene cfr Antimicrob Agents Chemother 55, 3714-3719.
- Vázquez-Laslop, N., Mankin, A.S. (2011) Picky nascent peptides do not talk to foreign ribosomes. Proc Natl Acad Sci USA 108, 5931-5932.
- Vázquez-Laslop, N., Klepacki, D., Mulhearn, D.C., Ramu, H., Krasnykh, O., Franzblau, S., Mankin, A.S. (2011) Role of antibiotic ligand in nascent peptide-dependent ribosome stalling. Proc Natl Acad Sci USA 108, 10496-10501.
- Vázquez-Laslop, N., Ramu, H., Mankin, A. S. (2011) Nascent peptide-mediated ribosome stalling promoted by antibiotics Ribosomes Structure Function and Dynamics 1, 377-392.
- Kannan, K., Mankin, A.S (2011) Macrolide antibiotics in the ribosome exit tunnel: species-specific binding and action Ann NY Acad Sci 1241, 33-47.
- Ramu, H., Vázquez-Laslop, N., Klepacki, D., Dai, Q., Piccirilli, J., Micura, R., Mankin, A.S. (2011) Nascent peptide in the ribosome exit tunnel affects functional properties of the A-Site of the peptidyl transferase center Mol Cell 41, 321-330.
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- Yan, F., LaMarre, J. M., Röhrich, R., Wiesner, J., Jomaa, H., Mankin, A. S., Fujimori, D. G. (2010) RlmN and Cfr are radical SAM enzymes involved in methylation of ribosomal RNA J Am Chem Soc 132, 3953-3964.
- Auerbach, T., Mermershtain, I., Davidovich, C., Bashan, A., Belousoff, M., Wekselman, I., Zimmerman, E., Xiong, L., Klepacki, D., Arakawa, K., Kinashi, H., Mankin, A.S., Yonath, A. (2010) The structure of ribosome-lankacidin complex reveals ribosomal sites for synergistic antibiotics. Proc Natl Acad Sci USA 107, 1983-1988.
- David-Eden, H., Mankin, A. S., Mandel-Gurfreund, Y. (2010) Structural signatures of antibiotic binding sites on the ribosome Nucl Acids Res 38, 5982-5994.
- Vázquez-Laslop, N., Ramu, H., Klepacki, D., Kannan, K., Mankin, A. S. (2010) The key function of a conserved and modified rRNA residue in the ribosomal response to the nascent peptide. EMBO J 29, 3108-3117.
- Llano-Sotelo, B., Dunkle, J., Klepacki, D., Zhang, W., Fernandes, P., Cate, J. H. D., Mankin, A. S. (2010) Binding and action of CEM-101, a new fluoroketolide antibiotic that inhibits protein synthesis Antimicrob Agents Chemother 54, 4961-4970.
- Dunkle, J. A., Xiong, L., Mankin, A. S., Cate, J. H. D. (2010) Structures of the Escherichia coli ribosome with antibiotics bound near the peptidyl transferase center explain spectra of drug action Proc Natl Acad Sci USA 107, 17152-17157.
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- T. Siibak, L. Peil, L. Xiong, A. Mankin, J. Remme, T. Tenson (2009) Erythromycin- and chloramphenicol-induced ribosomal assembly defects are secondary effects of protein synthesis inhibition Antimicrob Agents Chemother 53, 563-571.
- H. Ramu, A. Mankin, N. Vazquez-Laslop (2009) Programmed drug-dependent ribosome stalling Mol Microbiol 71, 811-824.
- Llano-Sotelo, B., Klepacki, D., Mankin, A.S. (2009) Selection of short peptides, inhibitors of translation J Mol Biol 391, 813-819.
- Llano-Sotelo, B., Hickerson, R. P., Lancaster, L., Noller, H.F., Mankin, A.S. (2009) Fluorescently labeled ribosomes as a tool for analyzing antibiotic binding RNA 15, 1597-1604.
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- N. Vazquez-Laslop, C. Thum, A. S. Mankin (2008) Molecular mechanism of drug-dependent ribosome stalling Mol Cell 30, 190-202.
- S. M. Toh, L. Xiong, T. Bae, A. S. Mankin (2008) The methyltransferase YfgB/RlmN is responsible for modification of adenosine 2503 in 23S rRNA RNA 14, 98-106.
- S. M. Toh, A. S. Mankin (2008) An indigenous posttranscriptional modification in the ribosomal peptidyl transferase center confers resistance to an array of protein synthesis inhibitors J Mol Biol 380, 593-597.
- L. K. Smith, A. S. Mankin (2008) Transcriptional and translational control of the mlr operon, which confers resistance to seven classes of protein synthesis inhibitors Antimicrob Agents Chemother 52, 1703-1712.
- S. Samant, H. Lee, M. Ghassemi, J. Chen, J. L. Cook, A. S. Mankin, A. A. Neyfakh (2008) Nucleotide biosynthesis is critical for growth of bacteria in human blood PLoS Pathog 4, e37.
- A. S. Mankin, N. Polacek (2008) SPARK: a new peptidyl transferase activity assay Methods Mol Med 142, 107-116.
- A. S. Mankin (2008) Macrolide myths Curr Opin Microbiol 11, 414-421.
- M. Bailey, T. Chettiath, A. S. Mankin (2008) Induction of erm(C) expression by noninducing antibiotics Antimicrob Agents Chemother 52, 866-874.
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- A. Yassin, A. S. Mankin (2007) Potential new antibiotic sites in the ribosome revealed by deleterious mutations in RNA of the large ribosomal subunit J Biol Chem 282, 24329-24342.
- S. M. Toh, L. Xiong, C. A. Arias, M. V. Villegas, K. Lolans, J. Quinn, A. S. Mankin (2007) Acquisition of a natural resistance gene renders a clinical strain of methicillin-resistant Staphylococcus aureus resistant to the synthetic antibiotic linezolid Mol Microbiol 64, 1506-1514.
- K. L. Leach, S. M. Swaney, J. R. Colca, W. G. McDonald, J. R. Blinn, L. M. Thomasco, R. C. Gadwood, D. Shinabarger, L. Xiong, A. S. Mankin (2007) The site of action of oxazolidinone antibiotics in living bacteria and in human mitochondria Mol Cell 26, 393-402.
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- T. Tenson, A. Mankin (2006) Antibiotics and the ribosome Mol Microbiol 59, 1664-1677.
- D. Sherman, L. Xiong, A. S. Mankin, A. Melman (2006) Synthesis and biological investigation of new 4”-malonyl tethered derivatives of erythromycin and clarithromycin Bioorg Med Chem Lett 16, 1506-1509.
- A. S. Mankin (2006) Nascent peptide in the “birth canal” of the ribosome Trends Biochem Sci 31, 11-13.
- A. Mankin (2006) Antibiotic blocks mRNA path on the ribosome Nat Struct Mol Biol 13, 858-860.
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- A. Yassin, K. Fredrick, A. S. Mankin (2005) Deleterious mutations in small subunit ribosomal RNA identify functional sites and potential targets for antibiotics Proc Natl Acad Sci U S A 102, 16620-16625.
- L. Xiong, Y. Korkhin, A. S. Mankin (2005) Binding site of the bridged macrolides in the Escherichia coli ribosome Antimicrob Agents Chemother 49, 281-288.
- N. Polacek, A. S. Mankin (2005) The ribosomal peptidyl transferase center: structure, function, evolution, inhibition Crit Rev Biochem Mol Biol 40, 285-311.
- B. A. Maguire, A. D. Beniaminov, H. Ramu, A. S. Mankin, R. A. Zimmermann (2005) A protein component at the heart of an RNA machine: the importance of protein l27 for the function of the bacterial ribosome Mol Cell 20, 427-435.
- M. D. Erlacher, K. Lang, N. Shankaran, B. Wotzel, A. Huttenhofer, R. Micura, A. S. Mankin, N. Polacek (2005) Chemical engineering of the peptidyl transferase center reveals an important role of the 2′-hydroxyl group of A2451 Nucleic Acids Res 33, 1618-1627.
- M. Beringer, C. Bruell, L. Xiong, P. Pfister, P. Bieling, V. I. Katunin, A. S. Mankin, E. C. Bottger, M. V. Rodnina (2005) Essential mechanisms in the catalysis of peptide bond formation on the ribosome J Biol Chem 280, 36065-36072.
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- K. A. Ruggero, L. K. Schroeder, P. C. Schreckenberger, A. S. Mankin, J. P. Quinn (2003) Nosocomial superinfections due to linezolid-resistant Enterococcus faecalis: evidence for a gene dosage effect on linezolid MICs Diagn Microbiol Infect Dis 47, 511-513.
- N. Polacek, M. J. Gomez, K. Ito, L. Xiong, Y. Nakamura, A. Mankin (2003) The critical role of the universally conserved A2602 of 23S ribosomal RNA in the release of the nascent peptide during translation termination Mol Cell 11, 103-112.
- M. Gaynor, A. S. Mankin (2003) Macrolide antibiotics: binding site, mechanism of action, resistance Curr Top Med Chem 3, 949-961.
- J. R. Colca, W. G. McDonald, D. J. Waldon, L. M. Thomasco, R. C. Gadwood, E. T. Lund, G. S. Cavey, W. R. Mathews, L. D. Adams, E. T. Cecil, J. D. Pearson, J. H. Bock, J. E. Mott, D. L. Shinabarger, L. Xiong, A. S. Mankin (2003) Cross-linking in the living cell locates the site of action of oxazolidinone antibiotics J Biol Chem 278, 21972-21979.
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- P. Sander, L. Belova, Y. G. Kidan, P. Pfister, A. S. Mankin, E. C. Bottger (2002) Ribosomal and non-ribosomal resistance to oxazolidinones: species-specific idiosyncrasy of ribosomal alterations Mol Microbiol 46, 1295-1304.
- N. Polacek, S. Swaney, D. Shinabarger, A. S. Mankin (2002) SPARK–a novel method to monitor ribosomal peptidyl transferase activity Biochemistry 41, 11602-11610.
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- L. Xiong, N. Polacek, P. Sander, E. C. Bottger, A. Mankin (2001) pKa of adenine 2451 in the ribosomal peptidyl transferase center remains elusive RNA 7, 1365-1369.
- T. Tenson, A. S. Mankin (2001) Short peptides conferring resistance to macrolide antibiotics Peptides 22, 1661-1668.
- N. Polacek, M. Gaynor, A. Yassin, A. S. Mankin (2001) Ribosomal peptidyl transferase can withstand mutations at the putative catalytic nucleotide Nature 411, 498-501.
- P. A. Mann, L. Xiong, A. S. Mankin, A. S. Chau, C. A. Mendrick, D. J. Najarian, C. A. Cramer, D. Loebenberg, E. Coates, N. J. Murgolo, F. M. Aarestrup, R. V. Goering, T. A. Black, R. S. Hare, P. M. McNicholas (2001) EmtA, a rRNA methyltransferase conferring high-level evernimicin resistance Mol Microbiol 41, 1349-1356.
- G. Garza-Ramos, L. Xiong, P. Zhong, A. Mankin (2001) Binding site of macrolide antibiotics on the ribosome: new resistance mutation identifies a specific interaction of ketolides with rRNA J Bacteriol 183, 6898-6907.
- M. Bocchetta, L. Xiong, S. Shah, A. S. Mankin (2001) Interactions between 23S rRNA and tRNA in the ribosomal E site RNA 7, 54-63.
- L. Belova, T. Tenson, L. Xiong, P. M. McNicholas, A. S. Mankin (2001) A novel site of antibiotic action in the ribosome: interaction of evernimicin with the large ribosomal subunit Proc Natl Acad Sci U S A 98, 3726-3731.
- A. S. Mankin (2001) Ribosomal Antibiotics Mol Biol 35, 509-520.
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- L. Xiong, P. Kloss, S. Douthwaite, N. M. Andersen, S. Swaney, D. L. Shinabarger, A. S. Mankin (2000) Oxazolidinone resistance mutations in 23S rRNA of Escherichia coli reveal the central region of domain V as the primary site of drug action J Bacteriol 182, 5325-5331.
- I. V. Velichutina, J. Dresios, J. Y. Hong, C. Li, A. Mankin, D. Synetos, S. W. Liebman (2000) Mutations in helix 27 of the yeast Saccharomyces cerevisiae 18S rRNA affect the function of the decoding center of the ribosome RNA 6, 1174-1184.
- S. I. Novikova, A. M. Bushueva, L. A. Trachuk, G. E. Konstantinova, A. V. Serkina, C. Hoischen, J. Gumpert, G. G. Chestukhina, A. Mankin, A. B. Shevelev (2000) Introduction of a mini-gene encoding a five-amino acid peptide confers erythromycin resistance on Bacillus subtilis and provides temporary erythromycin protection in Proteus mirabilis FEMS Microbiol Lett 182, 213-218.
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- L. Xiong, S. Shah, P. Mauvais, A. S. Mankin (1999) A ketolide resistance mutation in domain II of 23S rRNA reveals the proximity of hairpin 35 to the peptidyl transferase centre Mol Microbiol 31, 633-639.
- T. Tenson, J. V. Herrera, P. Kloss, G. Guarneros, A. S. Mankin (1999) Inhibition of translation and cell growth by minigene expression J Bacteriol 181, 1617-1622.
- A. S. Mankin, S. W. Liebman (1999) Baby, don’t stop! Nat Genet 23, 8-10.
- P. Kloss, L. Xiong, D. L. Shinabarger, A. S. Mankin (1999) Resistance mutations in 23 S rRNA identify the site of action of the protein synthesis inhibitor linezolid in the ribosomal peptidyl transferase center J Mol Biol 294, 93-101.
- P. Khaitovich, T. Tenson, A. S. Mankin, R. Green (1999) Peptidyl transferase activity catalyzed by protein-free 23S ribosomal RNA remains elusive RNA 5, 605-608.
- P. Khaitovich, T. Tenson, P. Kloss, A. S. Mankin (1999) Reconstitution of functionally active Thermus aquaticus large ribosomal subunits with in vitro-transcribed rRNA Biochemistry 38, 1780-1788.
- P. Khaitovich, A. S. Mankin, R. Green, L. Lancaster, H. F. Noller (1999) Characterization of functionally active subribosomal particles from Thermus aquaticus Proc Natl Acad Sci U S A 96, 85-90.
- P. Khaitovich, A. S. Mankin (1999) Effect of antibiotics on large ribosomal subunit assembly reveals possible function of 5 S rRNA J Mol Biol 291, 1025-1034.
- N. S. Chernyaeva, E. J. Murgola, A. S. Mankin (1999) Suppression of nonsense mutations induced by expression of an RNA complementary to a conserved segment of 23S rRNA J Bacteriol 181, 5257-5262.
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- S. Tripathi, P. S. Kloss, A. S. Mankin (1998) Ketolide resistance conferred by short peptides J Biol Chem 273, 20073-20077.
- B. T. Porse, I. Leviev, A. S. Mankin, R. A. Garrett (1998) The antibiotic thiostrepton inhibits a functional transition within protein L11 at the ribosomal GTPase centre J Mol Biol 276, 391-404.
- M. Bocchetta, L. Xiong, A. S. Mankin (1998) 23S rRNA positions essential for tRNA binding in ribosomal functional sites Proc Natl Acad Sci U S A 95, 3525-3530.
- A. L. Arkov, A. Mankin, E. J. Murgola (1998) An rRNA fragment and its antisense can alter decoding of genetic information J Bacteriol 180, 2744-2748.
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- T. Tenson, A. DeBlasio, A. Mankin (1996) A functional peptide encoded in the Escherichia coli 23S rRNA Proc Natl Acad Sci U S A 93, 5641-5646.
- G. T. Tan, A. DeBlasio, A. S. Mankin (1996) Mutations in the peptidyl transferase center of 23 S rRNA reveal the site of action of sparsomycin, a universal inhibitor of translation J Mol Biol 261, 222-230.
- M. Dam, S. Douthwaite, T. Tenson, A. S. Mankin (1996) Mutations in domain II of 23 S rRNA facilitate translation of a 23 S rRNA-encoded pentapeptide conferring erythromycin resistance J Mol Biol 259, 1-6.
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- T. Tenson, A. Mankin (1995) Comparison of functional peptide encoded in the Escherichia coli 23S rRNA with other peptides involved in cis-regulation of translation Biochem Cell Biol 73, 1061-1070.
- H. F. Noller, R. Green, G. Heilek, V. Hoffarth, A. Huttenhofer, S. Joseph, I. Lee, K. Lieberman, A. Mankin, C. Merryman, et al. (1995) Structure and function of ribosomal RNA Biochem Cell Biol 73, 997-1009.
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- Method of Screening to Find New Antibiotics. U.S. Patent 5,958,695 (1999). Inventor: Alexander Mankin
- Mapping New Sites of Antibiotic Action in the Ribosome. International Publication Number WO 2006/121703. Inventors: Alexander Mankin and Aymen Yassin